Boosted expression of the SARS-CoV nucleocapsid protein in tobacco and its immunogenicity in mice
Identifieur interne : 002F44 ( Main/Exploration ); précédent : 002F43; suivant : 002F45Boosted expression of the SARS-CoV nucleocapsid protein in tobacco and its immunogenicity in mice
Auteurs : NUOYAN ZHENG [République populaire de Chine] ; RAN XIA [République populaire de Chine] ; CUIPING YANG [République populaire de Chine] ; BOJIAO YIN [République populaire de Chine] ; YIN LI [République populaire de Chine] ; CHENGGUO DUAN [République populaire de Chine] ; LIMING LIANG [République populaire de Chine] ; HUISHAN GUO [République populaire de Chine] ; QI XIE [République populaire de Chine]Source :
- Vaccine [ 0264-410X ] ; 2009.
Descripteurs français
- Pascal (Inist)
- Wicri :
- topic : Vaccin.
English descriptors
- KwdEn :
Abstract
Vaccines produced in plant systems are safe and economical; however, the extensive application of plant-based vaccines is mainly hindered by low expression levels of heterologous proteins in plant systems. Here, we demonstrated that the post-transcriptional gene silencing suppressor p19 protein from tomato bushy stunt virus substantially enhanced the transient expression of recombinant SARS-CoV nucleo-capsid (rN) protein in Nicotiana benthamiana. The rN protein in the agrobacteria-infiltrated plant leaf accumulated up to a concentration of 79 μg per g fresh leaf weight at 3 days post infiltration. BALB/c mice were intraperitoneally vaccinated with pre-treated plant extract emulsified in Freund's adjuvant. The rN protein-specific IgG in the mouse sera attained a titer about 1:1,800 following three doses of immunization, which suggested effective B-cell maturation and differentiation in mice. Antibodies of the subclasses IgG1 and IgG2a were abundantly present in the mouse sera. During vaccination ofrN protein, the expression of IFN-γ and IL-10 was evidently up-regulated in splenocytes at different time points, while the expression of IL-2 and IL-4 was not. Up to now, this is the first study that plant-expressed recombinant SARS-CoV N protein can induce strong humoral and cellular responses in mice.
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream PascalFrancis, to step Corpus: 000196
- to stream PascalFrancis, to step Curation: 000792
- to stream PascalFrancis, to step Checkpoint: 000227
- to stream Main, to step Merge: 003004
- to stream Main, to step Curation: 002F44
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en" level="a">Boosted expression of the SARS-CoV nucleocapsid protein in tobacco and its immunogenicity in mice</title><author><name sortKey="Nuoyan Zheng" sort="Nuoyan Zheng" uniqKey="Nuoyan Zheng" last="Nuoyan Zheng">NUOYAN ZHENG</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Datun Road</s1><s2>Beijing 100101</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>4 aut.</sZ><sZ>9 aut.</sZ></inist:fA14><country>République populaire de Chine</country><placeName><settlement type="city">Pékin</settlement></placeName></affiliation><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>State Key Laboratory for Biocontrol, Sun Yat-sen (Zhongshan) University, 135 Xingang Road W</s1><s2>Guangzhou 510275</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>7 aut.</sZ></inist:fA14><country>République populaire de Chine</country><placeName><settlement type="city">Jiangmen</settlement><region type="province">Guangdong</region></placeName></affiliation></author><author><name sortKey="Ran Xia" sort="Ran Xia" uniqKey="Ran Xia" last="Ran Xia">RAN XIA</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Datun Road</s1><s2>Beijing 100101</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>4 aut.</sZ><sZ>9 aut.</sZ></inist:fA14><country>République populaire de Chine</country><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Cuiping Yang" sort="Cuiping Yang" uniqKey="Cuiping Yang" last="Cuiping Yang">CUIPING YANG</name><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>State Key Laboratory for Biocontrol, Sun Yat-sen (Zhongshan) University, 135 Xingang Road W</s1><s2>Guangzhou 510275</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>7 aut.</sZ></inist:fA14><country>République populaire de Chine</country><placeName><settlement type="city">Jiangmen</settlement><region type="province">Guangdong</region></placeName></affiliation></author><author><name sortKey="Bojiao Yin" sort="Bojiao Yin" uniqKey="Bojiao Yin" last="Bojiao Yin">BOJIAO YIN</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Datun Road</s1><s2>Beijing 100101</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>4 aut.</sZ><sZ>9 aut.</sZ></inist:fA14><country>République populaire de Chine</country><placeName><settlement type="city">Pékin</settlement></placeName></affiliation><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>State Key Laboratory for Biocontrol, Sun Yat-sen (Zhongshan) University, 135 Xingang Road W</s1><s2>Guangzhou 510275</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>7 aut.</sZ></inist:fA14><country>République populaire de Chine</country><placeName><settlement type="city">Jiangmen</settlement><region type="province">Guangdong</region></placeName></affiliation></author><author><name sortKey="Yin Li" sort="Yin Li" uniqKey="Yin Li" last="Yin Li">YIN LI</name><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>State Key Laboratory for Biocontrol, Sun Yat-sen (Zhongshan) University, 135 Xingang Road W</s1><s2>Guangzhou 510275</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>7 aut.</sZ></inist:fA14><country>République populaire de Chine</country><placeName><settlement type="city">Jiangmen</settlement><region type="province">Guangdong</region></placeName></affiliation></author><author><name sortKey="Chengguo Duan" sort="Chengguo Duan" uniqKey="Chengguo Duan" last="Chengguo Duan">CHENGGUO DUAN</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences</s1><s2>Beijing 100080</s2><s3>CHN</s3><sZ>6 aut.</sZ><sZ>8 aut.</sZ></inist:fA14><country>République populaire de Chine</country><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Liming Liang" sort="Liming Liang" uniqKey="Liming Liang" last="Liming Liang">LIMING LIANG</name><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>State Key Laboratory for Biocontrol, Sun Yat-sen (Zhongshan) University, 135 Xingang Road W</s1><s2>Guangzhou 510275</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>7 aut.</sZ></inist:fA14><country>République populaire de Chine</country><placeName><settlement type="city">Jiangmen</settlement><region type="province">Guangdong</region></placeName></affiliation></author><author><name sortKey="Huishan Guo" sort="Huishan Guo" uniqKey="Huishan Guo" last="Huishan Guo">HUISHAN GUO</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences</s1><s2>Beijing 100080</s2><s3>CHN</s3><sZ>6 aut.</sZ><sZ>8 aut.</sZ></inist:fA14><country>République populaire de Chine</country><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Qi Xie" sort="Qi Xie" uniqKey="Qi Xie" last="Qi Xie">QI XIE</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Datun Road</s1><s2>Beijing 100101</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>4 aut.</sZ><sZ>9 aut.</sZ></inist:fA14><country>République populaire de Chine</country><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">INIST</idno><idno type="inist">09-0341369</idno><date when="2009">2009</date><idno type="stanalyst">PASCAL 09-0341369 INIST</idno><idno type="RBID">Pascal:09-0341369</idno><idno type="wicri:Area/PascalFrancis/Corpus">000196</idno><idno type="wicri:Area/PascalFrancis/Curation">000792</idno><idno type="wicri:Area/PascalFrancis/Checkpoint">000227</idno><idno type="wicri:explorRef" wicri:stream="PascalFrancis" wicri:step="Checkpoint">000227</idno><idno type="wicri:doubleKey">0264-410X:2009:Nuoyan Zheng:boosted:expression:of</idno><idno type="wicri:Area/Main/Merge">003004</idno><idno type="wicri:Area/Main/Curation">002F44</idno><idno type="wicri:Area/Main/Exploration">002F44</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">Boosted expression of the SARS-CoV nucleocapsid protein in tobacco and its immunogenicity in mice</title><author><name sortKey="Nuoyan Zheng" sort="Nuoyan Zheng" uniqKey="Nuoyan Zheng" last="Nuoyan Zheng">NUOYAN ZHENG</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Datun Road</s1><s2>Beijing 100101</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>4 aut.</sZ><sZ>9 aut.</sZ></inist:fA14><country>République populaire de Chine</country><placeName><settlement type="city">Pékin</settlement></placeName></affiliation><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>State Key Laboratory for Biocontrol, Sun Yat-sen (Zhongshan) University, 135 Xingang Road W</s1><s2>Guangzhou 510275</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>7 aut.</sZ></inist:fA14><country>République populaire de Chine</country><placeName><settlement type="city">Jiangmen</settlement><region type="province">Guangdong</region></placeName></affiliation></author><author><name sortKey="Ran Xia" sort="Ran Xia" uniqKey="Ran Xia" last="Ran Xia">RAN XIA</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Datun Road</s1><s2>Beijing 100101</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>4 aut.</sZ><sZ>9 aut.</sZ></inist:fA14><country>République populaire de Chine</country><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Cuiping Yang" sort="Cuiping Yang" uniqKey="Cuiping Yang" last="Cuiping Yang">CUIPING YANG</name><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>State Key Laboratory for Biocontrol, Sun Yat-sen (Zhongshan) University, 135 Xingang Road W</s1><s2>Guangzhou 510275</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>7 aut.</sZ></inist:fA14><country>République populaire de Chine</country><placeName><settlement type="city">Jiangmen</settlement><region type="province">Guangdong</region></placeName></affiliation></author><author><name sortKey="Bojiao Yin" sort="Bojiao Yin" uniqKey="Bojiao Yin" last="Bojiao Yin">BOJIAO YIN</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Datun Road</s1><s2>Beijing 100101</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>4 aut.</sZ><sZ>9 aut.</sZ></inist:fA14><country>République populaire de Chine</country><placeName><settlement type="city">Pékin</settlement></placeName></affiliation><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>State Key Laboratory for Biocontrol, Sun Yat-sen (Zhongshan) University, 135 Xingang Road W</s1><s2>Guangzhou 510275</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>7 aut.</sZ></inist:fA14><country>République populaire de Chine</country><placeName><settlement type="city">Jiangmen</settlement><region type="province">Guangdong</region></placeName></affiliation></author><author><name sortKey="Yin Li" sort="Yin Li" uniqKey="Yin Li" last="Yin Li">YIN LI</name><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>State Key Laboratory for Biocontrol, Sun Yat-sen (Zhongshan) University, 135 Xingang Road W</s1><s2>Guangzhou 510275</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>7 aut.</sZ></inist:fA14><country>République populaire de Chine</country><placeName><settlement type="city">Jiangmen</settlement><region type="province">Guangdong</region></placeName></affiliation></author><author><name sortKey="Chengguo Duan" sort="Chengguo Duan" uniqKey="Chengguo Duan" last="Chengguo Duan">CHENGGUO DUAN</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences</s1><s2>Beijing 100080</s2><s3>CHN</s3><sZ>6 aut.</sZ><sZ>8 aut.</sZ></inist:fA14><country>République populaire de Chine</country><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Liming Liang" sort="Liming Liang" uniqKey="Liming Liang" last="Liming Liang">LIMING LIANG</name><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>State Key Laboratory for Biocontrol, Sun Yat-sen (Zhongshan) University, 135 Xingang Road W</s1><s2>Guangzhou 510275</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>7 aut.</sZ></inist:fA14><country>République populaire de Chine</country><placeName><settlement type="city">Jiangmen</settlement><region type="province">Guangdong</region></placeName></affiliation></author><author><name sortKey="Huishan Guo" sort="Huishan Guo" uniqKey="Huishan Guo" last="Huishan Guo">HUISHAN GUO</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences</s1><s2>Beijing 100080</s2><s3>CHN</s3><sZ>6 aut.</sZ><sZ>8 aut.</sZ></inist:fA14><country>République populaire de Chine</country><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Qi Xie" sort="Qi Xie" uniqKey="Qi Xie" last="Qi Xie">QI XIE</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Datun Road</s1><s2>Beijing 100101</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>4 aut.</sZ><sZ>9 aut.</sZ></inist:fA14><country>République populaire de Chine</country><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author></analytic><series><title level="j" type="main">Vaccine</title><title level="j" type="abbreviated">Vaccine</title><idno type="ISSN">0264-410X</idno><imprint><date when="2009">2009</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">Vaccine</title><title level="j" type="abbreviated">Vaccine</title><idno type="ISSN">0264-410X</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Cytokine</term><term>Humoral immunity</term><term>Immunogenicity</term><term>Mouse</term><term>Nicotiana tabacum</term><term>Nucleocapsid</term><term>Protein</term><term>Severe acute respiratory syndrome virus</term><term>Vaccine</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Virus syndrome respiratoire aigu sévère</term><term>Nicotiana tabacum</term><term>Souris</term><term>Nucléocapside</term><term>Protéine</term><term>Immunogénicité</term><term>Vaccin</term><term>Immunité humorale</term><term>Cytokine</term></keywords><keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Vaccin</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Vaccines produced in plant systems are safe and economical; however, the extensive application of plant-based vaccines is mainly hindered by low expression levels of heterologous proteins in plant systems. Here, we demonstrated that the post-transcriptional gene silencing suppressor p19 protein from tomato bushy stunt virus substantially enhanced the transient expression of recombinant SARS-CoV nucleo-capsid (rN) protein in Nicotiana benthamiana. The rN protein in the agrobacteria-infiltrated plant leaf accumulated up to a concentration of 79 μg per g fresh leaf weight at 3 days post infiltration. BALB/c mice were intraperitoneally vaccinated with pre-treated plant extract emulsified in Freund's adjuvant. The rN protein-specific IgG in the mouse sera attained a titer about 1:1,800 following three doses of immunization, which suggested effective B-cell maturation and differentiation in mice. Antibodies of the subclasses IgG1 and IgG2a were abundantly present in the mouse sera. During vaccination ofrN protein, the expression of IFN-γ and IL-10 was evidently up-regulated in splenocytes at different time points, while the expression of IL-2 and IL-4 was not. Up to now, this is the first study that plant-expressed recombinant SARS-CoV N protein can induce strong humoral and cellular responses in mice.</div></front></TEI><affiliations><list><country><li>République populaire de Chine</li></country><region><li>Guangdong</li></region><settlement><li>Jiangmen</li><li>Pékin</li></settlement></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Nuoyan Zheng" sort="Nuoyan Zheng" uniqKey="Nuoyan Zheng" last="Nuoyan Zheng">NUOYAN ZHENG</name></noRegion><name sortKey="Bojiao Yin" sort="Bojiao Yin" uniqKey="Bojiao Yin" last="Bojiao Yin">BOJIAO YIN</name><name sortKey="Bojiao Yin" sort="Bojiao Yin" uniqKey="Bojiao Yin" last="Bojiao Yin">BOJIAO YIN</name><name sortKey="Chengguo Duan" sort="Chengguo Duan" uniqKey="Chengguo Duan" last="Chengguo Duan">CHENGGUO DUAN</name><name sortKey="Cuiping Yang" sort="Cuiping Yang" uniqKey="Cuiping Yang" last="Cuiping Yang">CUIPING YANG</name><name sortKey="Huishan Guo" sort="Huishan Guo" uniqKey="Huishan Guo" last="Huishan Guo">HUISHAN GUO</name><name sortKey="Liming Liang" sort="Liming Liang" uniqKey="Liming Liang" last="Liming Liang">LIMING LIANG</name><name sortKey="Nuoyan Zheng" sort="Nuoyan Zheng" uniqKey="Nuoyan Zheng" last="Nuoyan Zheng">NUOYAN ZHENG</name><name sortKey="Qi Xie" sort="Qi Xie" uniqKey="Qi Xie" last="Qi Xie">QI XIE</name><name sortKey="Ran Xia" sort="Ran Xia" uniqKey="Ran Xia" last="Ran Xia">RAN XIA</name><name sortKey="Yin Li" sort="Yin Li" uniqKey="Yin Li" last="Yin Li">YIN LI</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SrasV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 002F44 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 002F44 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= SrasV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= Pascal:09-0341369
|texte= Boosted expression of the SARS-CoV nucleocapsid protein in tobacco and its immunogenicity in mice
}}
| This area was generated with Dilib version V0.6.33. |  |